Aristidis Charonis, Biomedical Research Foundation of the Academy of Athens (BRFAA)

Additional PIs: Effie Tsilibary (N.C.S.R. Demokritos), Garyfallia Drosopoulou (N.C.S.R. Demokritos), Antonia Vlahou (Biomedical Research Foundation of the Academy of Athens)

Research Focus:

Mechanisms and markers of renal fibrosis as proposed from proteomic studies and RNA-seq studies; evaluation of the proteins selected, their role and their validity as markers; evaluation of long non-coding RNAs (Charonis)

Regulation of expression of differentiation markers in podocytes in Chronic Kidney Disease (Tsilibary)

Effect of hyperglycemia and vitamin D analog administration on podocyte phenotype (Drosopoulou)

Urine markers for the detection of Chronic Kidney Disease (Vlahou)

Relevance of your Provisional grouping in KidneyConnect:

The groups of Drs. Tsilibary and Drosopoulou are focusing on the regulation of podocalyxin, nephrin and interacting proteins (CD2AP, podocin) under normal and high glucose conditions; also on the nephroprotective role of vitamin D and its analogs via these macromolecules and the podocyte survival signaling pathways under the above conditions. In the future, work carried on in vitro will be also repeated in animal models of diabetes with emphasis on Zucker rats and db/db mice where sudies with microarrays and proteomics are anticipated.

The groups of Drs. Charonis and Vlahou are focusing on proteomic analysis of renal changes in the UUO model in rats. Three proteins, calreticulin, transgelin and the 14-3-3 family of proteins are observed to exhibit altered expression at early stages. Proteomics are being used to understand the cellular alterations of the specific renal cell types that exhibit these changes and transcription factors involved are being studied. Phenotypic alterations are examined and mechanisms of altered expression are also explored. In addition, the role of long non-coding RNAs in the development of renal fibrosis is examined.

Resources Available relevant to your grouping:

  • Special Techniques: Proteomic studies using tissue sections subjected to laser capture microdissection and imaging mass spectroscopy (Vlahou)
  • Animal Models/Knock Outs: calreticulin +/- mice (from Dr. Michalak, Edmonton, Canada)
  • Datasets: data from RNA-seq analysis of renal fractions taken from days 0, 2 and 8 post-ligation in the Unilateral Ureteric Obstructio (UUO) model in mice.

Clinical/Translational Resources:

  • Biobanks
  • Population/Patient Cohorts
  • Special Techniques for Clinical Assessment
  • Patient Groups for Clinical trials
  • Others