Giuseppe Remuzzi, Istituto di Ricerche Farmacologiche Mario Negri

Additional PIs: Norberto Perico, Giorgio Gentile

Research Focus: experimental and clinical nephrology, immunology, organ transplantation, genetics of rare diseases

Relevance of your Provisional grouping in KidneyConnect:

Our group has a long-lasting experience in traslational research that justifies our current provisional grouping in KidneyConnect in the related section. Indeed a major characteristics of our Institution is to bridge from discovery via animal models to clinical application, and back to models to address any specific issue/hypothesis possibly emerged from the clinical studies. This approach implies that we have full translational resources that include murine/rat models of chronic nephropathies and transplantation and related patient cohorts for trans-species validation, comprehensive renal histology libraries, clinical dataset and stored samples.

Resources Available relevant to your grouping:

  • Special Techniques:
    Digital image processing and quantification of three-dimensional structures, PCR, real time PCR, FACS analysis and FACS sorter, purification of proteins, next generation sequencing by Sanger sequencers and a NGS (Ion Torrent). The laboratories are also equipped with the latest equipment for cutting-edge technology that will have an infrastructure based on computer systems for data collection and analysis and combined data and image processing.

  • Animal Models/Knock Outs:
    Non diabetic proteinuric disease
    Passive Heymann nephritis (rat)
    Renal mass reduction (rat)
    Adriamycin-induced nephropathy (rat and mouse)
    Protein overload proteinuria (mouse)
    Lupus nephritis (NZBxNZW mice)

  • Diabetic nephropathies
    Type II: Zucker Diabetic Fatty rats
    Type II: BTBRob/ob mice
    Type I: streptozotocin-induced diabetes (rat and mouse)

  • Polycystic kidney disease

  • PCK rats

  • Acute kidney injury

  • Cisplatin-induced acute renal injury (mouse)

  • Kidney transplantation

  • Acute cellular rejection
    (Balb/c  C57/B16 mice)
    (Brown Norway Lewis rats)

  • Chronic allograft rejection
    (Wistar Furth  Lewis rats)

Datasets:

Electronic databases with clinical, hematochemical, and (in some specific case) genetic information of individual patients with chronic nephropathy or kidney transplant enrolled in small or large clinical trials are available

Others:

  • Clinical/Translational Resources
  • Biobanks: DNA and samples from chronic kidney diseases, type 2 diabetes and rare kidney diseases patients

  • Population/Patient Cohorts:
  • BENEDICT and DEMAND cohorts including hypertensive patients with type 2 diabetes with or without diabetic nephropathy
  • VALID cohort including type 2 diabetes and overt nephropathy
  • VARIETY cohort including hypertensive patients with type 2 diabetes and high-normal albuminuria
  • DIABASI high-risk subjects with type 2 diabetes (WHO criteria) and high blood pressure
  • ALADIN cohort including Autosomal Dominant Polycystic Kidney Disease patients
  • CRESO cohorts including Subjects with Abdominal Obesity and Type 2 Diabetes at Increased Renal and Cardiovascular Risk.
  • ARCADIA and CINDY cohorts including patients on hemodialysis   
  • ATHENA cohort including kidney transplanted patients              
  • FABRY cohort including Fabry patients with renal involvement
  • ALPORT cohort including Alport patients with renal involvement
  • Special Techniques for Clinical Assessment:
    GFR measurement by Iohexol Clearance, Hyperinsulinemic-euglicemic clamp, Imaging Analysis of CT and Magnetic Resonance Images, Biopsies, Tonometry.

  • Patient Groups for Clinical trials:
    Type 2 diabetes, hypertension, obesity, chronic nephropathies (normo- and micro-albuminuria and overt nephropathy), Autosomal Dominant Polycystic Kidney Disease (ADPKD), Autosomal Dominant Familial form of Focal Segmental Glomerulosclerosis (FSGS), nephrotic syndrome.

Others:

Within the Mario Negri Institute a clinical facility, the Clinical Research Centre for Rare Diseases Aldo & Cele Daccò has been established specifically aimed at the application of basic research into clinical practice, through clinical studies. Its activities are mainly focused on the study of the mechanisms of progression of chronic nephropathies, of new prevention and intervention strategies for diabetic nephropathy, non diabetic chronic nephropathies, chronic allograft dysfunction, of cardiovascular complications of diabetes, chronic renal disease, dialysis and transplantation and of thrombotic microangiopathies. The Center has all the facilities in term of personnel (biologists, physicians, pharmacologists, bioengineers, informatics, nurses, geneticists, and technicians), techniques and apparatus to performing clinical trials.

The Centre leads the International Registry of Recurrent and Familial HUS and TTP that has collected more than 1000 cases of atypical HUS and TTP referred from 100 Italian and 80 European and extra-European Centres. The Center also coordinates the Italian Registries of MPGN, with more than 200 patients with DDD, MPGN I and C3GN, and of SRNS with 240 patients.